The Guided Tour of Metalloproteins
Bob Morris of the University of Toronto created this website when he was teaching a class on Bioinorganic Chemistry.
Bob Morris of the University of Toronto created this website when he was teaching a class on Bioinorganic Chemistry.
This learning object focuses on the new video series, “Voices in Inorganic Chemistry,” established to commemorate the 50th anniversary of the American Chemical Society journal, Inorganic Chemistry. The are currently 12 videos celebrating pioneers in the field of inorganic chemistry. This activity consists of two components, namely the students watching one interview and writing an essay about their chosen inorganic chemist.
Determining the reactive intermediates in metalloenzymes is a very involved task, and requires drawing from many different spectroscopies and physical methods. The facile activation and oxidation of methane to produce methanol is one of the "holy grails" of inorganic chemistry. Strategies exist within materials science and organometallic chemistry to activate methane, but using the enzyme methane monooxygenase, nature is able to carry out this difficult reaction at ambient temperatures and pressures (and in water, too!).
This lecture provides a short introduction to the other half of biological iron chemistry: enzymes that do not contain a porphyrin group that ligates the iron atom. There are several important applications for non-heme iron in cells, both mammalian and bacterial. Oxygen activating non-heme iron enzymes fall into a few basic categories and includes mononuclear iron monooxygenases and dioxygenases, and binuclear iron monooxygenases. The requirements to activate and utilize dioxygen will be given.
As a non-bioinorganic chemist, I am always looking for resources to help me teach bioinorganic chemistry in both my sophomore-level and advanced inorganic chemistry courses. The "metalloproteome" was the subject of an article in the December 12, 2011 issue of C&E News ("Merging Metals into Proteomics: Tackling the Systematic Study of Metalloproteins"). In this article, the author mentions a new database, called Metal MACiE, of metals in metalloenzymes.
This is an in class exercise that I use to emphasize the need for metal ion transport and storage in biochemistry. Applying the Van't Hoff equation to the Ksp value at 25°C for ferric hydroxide, students calculate the iron concentration at which ferric hydroxide would begin to precipitate out in the blood. It' s an interesting problem that requires very little math beyond that used in gen chem, and the answer is in stark contrast to the amount of iron that we actually store in our bodies.
This screencast is a brief introduction to some of the features of VIPEr.
A little more than 5 slides, this is a video I made for a colleague to use in General Chemistry as an intro, or hook, into exciting topics in chemistry (in this case, bioinorganic). I use these slides as an intro to my junior/senior Inorganic course on the first day of class, to ask the question "What is Inorganic Chemistry?" and get them to think about the "living" parts of "inorganic". Topics include an overview of essential, toxic, and medicinally active elements of the periodic table, key examples of metalloprotein active sites, and an overview of the functional roles of biological in
In 2011, I was fortunate to have Nicolai Lehnert come and speak to my bioinorganic class on his work modeling the FeB (non heme iron) center in bacterial Nitric Oxide reductase. He suggested this paper to prepare the students for his talk and I developed this reading guide to help them (the students) get more out of the reading.
This is a Reading guide to the Review article Transition Metal Speciation in the Cell: Insights from the Chemistry of Metal Ion Receptors Lydia A. Finney, et al. Science 300, 931 (2003);
DOI: 10.1126/science.1085049.